Interleukin 1 beta-induced increase in substance P in rat myenteric plexus

Gastroenterology. 1993 Dec;105(6):1754-60. doi: 10.1016/0016-5085(93)91073-q.


Background: Substance P (SP) is increased in the inflamed intestine of Trichinella spiralis-infected rats, but the underlying mechanism is unknown. Interleukin 1 beta (IL-1 beta) messenger RNA and protein is expressed in the longitudinal muscle-myenteric plexus (LM-MP) of this model. Thus, the purpose of the study was to examine the ability of human recombinant IL-1 beta (hrIL-1 beta) to increase SP in LM-MP preparations from the intestine of noninfected rats.

Methods: LM-MP preparations were incubated with hrIL-1 beta, and immunoreactive SP (IR-SP) was assessed in the tissues by radioimmunoassay or immunohistochemistry.

Results: hrIL-1 beta increased IR-SP in the tissue in a time- and concentration-dependent manner, being maximal after 6 hours at a concentration of 10 ng/mL. The IR-SP could be depleted by scorpion venom, and immunohistochemistry revealed increased staining for SP within nerves of the LM-MP. The action of IL-1 beta was dependent on protein synthesis, was receptor mediated, and was not due to endotoxin contamination of the cytokine preparation.

Conclusions: hrIL-1 beta stimulates the synthesis of SP in myenteric nerves of rat intestine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dose-Response Relationship, Drug
  • In Vitro Techniques
  • Interleukin-1 / pharmacology*
  • Male
  • Myenteric Plexus / drug effects
  • Myenteric Plexus / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Proteins / pharmacology
  • Substance P / analysis
  • Substance P / biosynthesis*


  • Interleukin-1
  • Recombinant Proteins
  • Substance P