Melanocyte lineage-specific antigens recognized by monoclonal antibodies NKI-beteb, HMB-50, and HMB-45 are encoded by a single cDNA

Am J Pathol. 1993 Dec;143(6):1579-85.


The glycoproteins recognized by monoclonal antibody (MAb) NKI-beteb are among the best diagnostic markers for human melanoma. MAb NKI-beteb reacts with melanoma cells throughout tumor development and does not cross-react with other tumor or normal cells, except for cells of the melanocytic lineage. Two other melanocyte lineage-specific MAbs, HMB-50 and HMB-45, show a specificity and staining pattern strikingly similar to the ones observed for NKI-beteb. Herein, we demonstrate that all three MAbs recognize protein products encoded by a single cDNA. Expression of this cDNA in BLM cells results in immunoreactivity with all three MAbs. In addition, we demonstrate co-distribution of the RNA species detected by the cDNA with the proteins recognized by the MAbs in tissue sections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • Biomarkers, Tumor / analysis
  • Blotting, Northern
  • Cell Line
  • DNA, Complementary / analysis*
  • DNA, Complementary / genetics
  • Epitopes / genetics*
  • Fluorescent Antibody Technique
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Melanocytes / cytology*
  • Melanocytes / immunology*
  • Melanoma / immunology*
  • Melanoma / pathology*
  • RNA / analysis
  • RNA / genetics
  • RNA, Neoplasm / analysis
  • RNA, Neoplasm / genetics
  • Skin / cytology
  • Skin / immunology
  • Skin / pathology
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • DNA, Complementary
  • Epitopes
  • RNA, Neoplasm
  • RNA