BDNF and NT-3 induce intracellular Ca2+ elevation in hippocampal neurones

Neuroreport. 1993 Sep 30;4(12):1303-6. doi: 10.1097/00001756-199309150-00004.

Abstract

The effects of neurotrophins on intracellular Ca2+ levels in rat hippocampal neurones were studied in vitro using fura-2 fluorescence microscopy. BDNF and NT-3, but not NGF, rapidly increased cytoplasmic Ca2+ concentrations in these neurones ten-fold to reach 1 microM. Moreover in some of the neurones both BDNF and NT-3 elicited Ca2+ responses, indicative of the presence of functional receptors for these neurotrophins in the same cell. In these cultures approximately 80% of the hippocampal neurones were stained with antibodies against full-length TrkB. The expression of functional TrkB was also confirmed by RNA analysis. These results demonstrate the presence of functional receptors for BDNF and NT-3 in hippocampal neurones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain-Derived Neurotrophic Factor
  • Calcium / metabolism*
  • Cells, Cultured
  • Female
  • Hippocampus / cytology
  • Hippocampus / drug effects
  • Hippocampus / metabolism*
  • Immunohistochemistry
  • Microscopy, Fluorescence
  • Nerve Growth Factors / pharmacology*
  • Nerve Tissue Proteins / pharmacology*
  • Neuronal Plasticity / drug effects
  • Neurons / metabolism*
  • Neurotrophin 3
  • Pregnancy
  • RNA / metabolism
  • Rats
  • Receptor Protein-Tyrosine Kinases / metabolism
  • Receptors, Nerve Growth Factor / drug effects
  • Receptors, Nerve Growth Factor / metabolism
  • Recombinant Proteins / metabolism

Substances

  • Brain-Derived Neurotrophic Factor
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • Neurotrophin 3
  • Receptors, Nerve Growth Factor
  • Recombinant Proteins
  • RNA
  • Receptor Protein-Tyrosine Kinases
  • Calcium