Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family

Cell. 1993 Dec 17;75(6):1169-78. doi: 10.1016/0092-8674(93)90326-l.


The Fas antigen (Fas) belongs to the tumor necrosis factor (TNF)/nerve growth factor receptor family, and it mediates apoptosis. Using a soluble form of mouse Fas, prepared by fusion with human immunoglobulin Fc, Fas ligand was detected on the cell surface of a cytotoxic T cell hybridoma, PC60-d10S. A cell population that highly expresses Fas ligand was sorted using a fluorescence-activated cell sorter, and its cDNA was isolated from the sorted cells by expression cloning. The amino acid sequence indicated that Fas ligand is a type II transmembrane protein that belongs to the TNF family. The recombinant Fas ligand expressed in COS cells induced apoptosis in Fas-expressing target cells. Northern hybridization revealed that Fas ligand is expressed in activated splenocytes and thymocytes, consistent with its involvement in T cell-mediated cytotoxicity and in several nonlymphoid tissues, such as testis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Surface / biosynthesis*
  • Antigens, Surface / genetics*
  • Apoptosis*
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Flow Cytometry
  • Gene Expression
  • Humans
  • Hybridomas
  • Immunoglobulin Fc Fragments / biosynthesis
  • Mice
  • Molecular Sequence Data
  • Multigene Family
  • RNA, Messenger / biosynthesis
  • Rats
  • Receptors, Cell Surface / biosynthesis
  • Recombinant Fusion Proteins / biosynthesis
  • Sequence Homology, Amino Acid
  • T-Lymphocytes, Cytotoxic
  • Transfection
  • Tumor Necrosis Factor-alpha / genetics*
  • fas Receptor


  • Antigens, Surface
  • DNA, Complementary
  • Immunoglobulin Fc Fragments
  • RNA, Messenger
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • Tumor Necrosis Factor-alpha
  • fas Receptor

Associated data

  • GENBANK/L20821
  • GENBANK/L20822
  • GENBANK/L20823
  • GENBANK/L20888
  • GENBANK/L20889
  • GENBANK/L33709
  • GENBANK/S60904
  • GENBANK/S60905
  • GENBANK/S60924
  • GENBANK/U03470