Expression cloning of a functional glycoprotein ligand for P-selectin

Cell. 1993 Dec 17;75(6):1179-86. doi: 10.1016/0092-8674(93)90327-m.


The initial adhesive interactions between circulating leukocytes and endothelia are mediated, in part, by P-selectin. We now report the expression cloning of a functional ligand for P-selectin from an HL-60 cDNA library. The predicted amino acid sequence reveals a novel mucin-like transmembrane protein. Significant binding of transfected COS cells to P-selectin requires coexpression of both the protein ligand and a fucosyltransferase. This binding is calcium dependent and can be inhibited by a neutralizing monoclonal antibody to P-selectin. Cotransfected COS cells express the ligand as a homodimer of 220 kd. A soluble ligand construct, when coexpressed with fucosyltransferase in COS cells, also mediates P-selectin binding and is immunocrossreactive with the major HL-60 glycoprotein that specifically binds P-selectin.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • CHO Cells
  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism
  • Cell Line
  • Cloning, Molecular
  • Cricetinae
  • DNA, Complementary / metabolism
  • Endothelium, Vascular / physiology
  • Gene Library
  • Humans
  • Leukemia, Promyelocytic, Acute
  • Leukocytes / physiology
  • Macromolecular Substances
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • P-Selectin
  • Platelet Membrane Glycoproteins / metabolism*
  • Sequence Homology, Amino Acid
  • Transfection
  • Tumor Cells, Cultured


  • Cell Adhesion Molecules
  • DNA, Complementary
  • Macromolecular Substances
  • Membrane Glycoproteins
  • P-Selectin
  • P-selectin ligand protein
  • Platelet Membrane Glycoproteins

Associated data

  • GENBANK/L20821
  • GENBANK/L20822
  • GENBANK/L20823
  • GENBANK/L20888
  • GENBANK/L20889
  • GENBANK/L33709
  • GENBANK/S60904
  • GENBANK/S60905
  • GENBANK/S60924
  • GENBANK/U02297