Abstract
An escape variant of Borrelia burgdorferi, selected with a monoclonal antibody to OspB, expressed a truncated form of OspB, the result of point mutations in the ospB gene leading to a premature termination codon. A single amino acid position in the C terminus of OspB was critical for monoclonal antibody recognition. The variations in the ospB gene suggest a mechanism for the evasion of the immune response by these organisms and may also have implications for current diagnostic and vaccine efforts.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antibodies, Bacterial / immunology
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Antibodies, Monoclonal / immunology
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / immunology
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Antigens, Surface / genetics*
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Bacterial Outer Membrane Proteins / genetics*
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Base Sequence
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Borrelia burgdorferi Group / genetics
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Borrelia burgdorferi Group / immunology*
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DNA Primers / chemistry
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Epitopes
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Genes, Bacterial
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Molecular Sequence Data
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Peptides / chemistry
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Peptides / immunology
Substances
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Antibodies, Bacterial
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Antibodies, Monoclonal
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Antigens, Bacterial
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Antigens, Surface
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Bacterial Outer Membrane Proteins
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DNA Primers
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Epitopes
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Peptides
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OspB protein, Borrelia burgdorferi
Associated data
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GENBANK/X74808
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GENBANK/X74809
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GENBANK/X74810