Growth-regulatory effects of sensory neuropeptides, epidermal growth factor, insulin, and somatostatin on the non-transformed intestinal epithelial cell line IEC-6 and the colon cancer cell line HT 29

Scand J Gastroenterol. 1993 Oct;28(10):879-84. doi: 10.3109/00365529309103129.


A non-transformed small-intestinal cell line from the rat (IEC-6) and a human colon cancer cell line (HT 29) were examined for their trophic response to sensory neuropeptides. Substance P, neurokinin A (NKA), calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP), and peptide YY (PYY) were tested. Epidermal growth factor (EGF), insulin, and somatostatin-14 were also used. Interaction studies were performed on IEC-6 cells by combining EGF or insulin with somatostatin-14. The sensory neuropeptides had no effect either on IEC-6 cell growth and DNA synthesis or on HT29 cell growth. EGF and insulin stimulated cell growth and DNA synthesis in IEC-6 cells and cell growth in HT 29 cells in a dose-dependent fashion. Somatostatin-14 had no effect either alone or in combination with EGF or insulin on IEC-6 cell growth and DNA synthesis. HT 29 cell growth was inhibited by somatostatin-14 only in the presence of serum with a maximal and significant response at 10(-7) M. Our observations suggest that the sensory neuropeptides do not exert a direct growth-regulatory effect either on IEC-6 cells or on HT 29 cells. Somatostatin, however, inhibits serum-induced HT 29 cell growth but does not interfere directly with the proliferative effect of serum, EGF, or insulin on IEC-6 cells in this model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitonin Gene-Related Peptide / pharmacology
  • Cell Division / drug effects
  • Cell Line, Transformed
  • Cell Transformation, Neoplastic / drug effects*
  • Cell Transformation, Neoplastic / metabolism
  • Cell Transformation, Neoplastic / pathology
  • Cells, Cultured
  • Colonic Neoplasms / metabolism
  • Colonic Neoplasms / pathology*
  • DNA, Neoplasm / biosynthesis
  • Dose-Response Relationship, Drug
  • Epidermal Growth Factor / pharmacology*
  • Epithelium / pathology
  • Gastrointestinal Hormones / pharmacology
  • Humans
  • Insulin / pharmacology*
  • Intestinal Mucosa / metabolism
  • Intestines / drug effects*
  • Intestines / pathology
  • Neurokinin A / pharmacology
  • Neurons, Afferent / metabolism
  • Neuropeptides / metabolism
  • Neuropeptides / pharmacology*
  • Peptide YY
  • Peptides / pharmacology
  • Rats
  • Somatostatin / pharmacology*
  • Substance P / pharmacology
  • Transforming Growth Factor beta / pharmacology
  • Vasoactive Intestinal Peptide / pharmacology


  • DNA, Neoplasm
  • Gastrointestinal Hormones
  • Insulin
  • Neuropeptides
  • Peptides
  • Transforming Growth Factor beta
  • Peptide YY
  • Substance P
  • Vasoactive Intestinal Peptide
  • Somatostatin
  • Epidermal Growth Factor
  • Neurokinin A
  • Calcitonin Gene-Related Peptide