Structure of the murine VCAM1 gene

Genomics. 1993 Nov;18(2):387-91. doi: 10.1006/geno.1993.1480.

Abstract

The architecture of the murine VCAM1 gene, encoding vascular cell adhesion molecule-1, was determined. its 10 exons span approximately 20 kb. Exon 1 encodes the 5' untranslated region and the signal peptide; exons 2-4 and 6-9, the C2 or H-type immunoglobulin domains; and exon 10, the transmembrane and cytoplasmic domains followed by the entire 3' untranslated region. All immunoglobulin-like domains are encoded by separate exons, and exon splice junctions occur after the first nucleotide of amino acid codons (type 1). Exon 5 encodes a novel domain unique to murine VCAM-1 and inclusion of this exon by alternative splicing results in a truncated three-immunoglobulin-like domain form, which is bound to the cell membrane by a phosphatidylinositol linkage at its carboxy terminus (encoded by exon 5). The murine VCAM1 core promoter contains a high degree of homology to the human, including conserved consensus binding sites for NF-kappa B, the Ets class, and the GATA family of transcription factors, suggesting that the murine and human VCAM1 genes may be under similar transcriptional control.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Adhesion Molecules / genetics*
  • DNA
  • Humans
  • Male
  • Mice
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Vascular Cell Adhesion Molecule-1

Substances

  • Cell Adhesion Molecules
  • Vascular Cell Adhesion Molecule-1
  • DNA

Associated data

  • GENBANK/L22301
  • GENBANK/L22349
  • GENBANK/L22350
  • GENBANK/L22351
  • GENBANK/L22352
  • GENBANK/L22353
  • GENBANK/L22354
  • GENBANK/L22355