Effects of interleukin-3 with or without the c-kit ligand, stem cell factor, on the survival and cytoplasmic granule formation of mouse basophils and mast cells in vitro

Am J Pathol. 1994 Jan;144(1):160-70.


We assessed the ultrastructure and the cell-surface expression of receptors for immunoglobulin E (Fc epsilon R), and c-kit, the receptor for stem cell factor (SCF), in mouse basophils and mast cells present in short-term cultures of mouse bone marrow cells in interleukin-3 (IL-3) with or without SCF. Basophils did not develop increased numbers of cytoplasmic granules and underwent apoptosis in cultures containing IL-3 and SCF, whereas mast cells thrived and developed increased numbers of granules. Basophils were nearly all Fc epsilon R+ c-kit- when sorted after culture in IL-3 and SCF; most mast cells were Fc epsilon R+ c-kit+. However, a second population of Fc epsilon R+ c-kit- mast cells was present after culture in IL-3 and SCF. These c-kit receptor-negative mast cells were less mature than c-kit+ mast cells and contained significantly fewer cytoplasmic granules than the c-kit+ mast cells present in the same cultures (P < 0.001). Thus, mouse basophils express little or no c-kit receptor on their surface, nor can they survive for long periods in SCF-supplemented cultures. By contrast, mouse mast cells seem to express the Fc epsilon R early in their development, even before they express detectable c-kit receptors on their surface. IL-3 promotes cytoplasmic granule formation in immature mast cells, but even more granules are formed when c-kit receptor-positive immature mast cells are cultured in both SCF and IL-3.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Basophils / cytology
  • Basophils / drug effects
  • Basophils / physiology*
  • Bone Marrow / metabolism
  • Bone Marrow / ultrastructure
  • Bone Marrow Cells
  • Cell Survival / drug effects
  • Cells, Cultured
  • Cytoplasmic Granules / physiology*
  • Female
  • Hematopoietic Cell Growth Factors / pharmacology*
  • Interleukin-3 / pharmacology*
  • Mast Cells / cytology
  • Mast Cells / drug effects
  • Mast Cells / physiology*
  • Mice
  • Stem Cell Factor
  • Stem Cells / cytology


  • Hematopoietic Cell Growth Factors
  • Interleukin-3
  • Stem Cell Factor