Correlation of the insulin receptor substrate-1 with insulin-responsive deoxyglucose transport in 3T3-L1 adipocytes

Biochem Biophys Res Commun. 1994 Jan 28;198(2):523-30. doi: 10.1006/bbrc.1994.1077.


We have devised procedures for decreasing the amount of IRS-1 in 3T3-L1 adipocytes (viz., chronic treatments with insulin, dexamethasone, 1-methyl-3-isobutylxanthine, cycloheximide or actinomycin D) and have determined the correlation between the amounts of IRS-1, insulin receptor, GluT4 and phosphatidylinositol 3'-kinase regulatory subunit with insulin-responsive dGlc transport. Each of these treatments decreased insulin responsiveness that correlated with the amount of IRS-1, but not with the amount of the other signaling proteins or tyrosine-phosphorylated IRS-1. Removal of insulin after chronic treatment resulted in a return of both insulin responsiveness and IRS-1. Increased expression of IRS-1 occurred during differentiation simultaneously with increased insulin-responsive dGlc transport. These data are consistent with a role of IRS-1 in insulin signaling to the glucose transport system.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Methyl-3-isobutylxanthine / pharmacology
  • 3T3 Cells
  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Animals
  • Biological Transport
  • Cell Differentiation
  • Cycloheximide / pharmacology
  • Dactinomycin / pharmacology
  • Deoxyglucose / pharmacokinetics*
  • Dexamethasone / pharmacology
  • Fibroblasts / physiology
  • Glucose Transporter Type 4
  • Insulin / metabolism
  • Insulin / pharmacology*
  • Insulin Receptor Substrate Proteins
  • Mice
  • Monosaccharide Transport Proteins / metabolism
  • Muscle Proteins*
  • Phosphatidylinositol 3-Kinases
  • Phosphoproteins / deficiency*
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Receptor, Insulin / metabolism
  • Signal Transduction*


  • Glucose Transporter Type 4
  • Insulin
  • Insulin Receptor Substrate Proteins
  • Irs1 protein, mouse
  • Monosaccharide Transport Proteins
  • Muscle Proteins
  • Phosphoproteins
  • Slc2a4 protein, mouse
  • Dactinomycin
  • Dexamethasone
  • Cycloheximide
  • Deoxyglucose
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor)
  • Receptor, Insulin
  • 1-Methyl-3-isobutylxanthine