Role of CD40 antigen and interleukin-2 in T cell-dependent human B lymphocyte growth

Eur J Immunol. 1994 Feb;24(2):330-5. doi: 10.1002/eji.1830240209.


In the present study, we examined the participation of CD40 ligand (L)-CD40 interaction in T cell-dependent B cell responses. To this end, purified B lymphocytes were cultured over irradiated CD4+ cloned T cells activated with immobilized anti-CD3 antibody. The anti-CD40 mAb 89 strongly blocked, in a specific fashion, both proliferation and Ig secretion of tonsil B cells. Interestingly, proliferation of surface (s)IgD+ B cell was significantly less inhibited by anti-CD40 than that of sIgD- cells. Preactivated T cells induced B cells to grow and secrete immunoglobulins preferentially in response to IL-2. This contrasts with the CD40 system where B cells are essentially responsive to IL-4 and IL-10 but not to IL-2 alone. Collectively, these data indicate that CD40L-CD40 interaction plays an important role in IL-2 mediated T cell-dependent B cell responses. However, the activation of a subset of sIgD+ cells may be independent of this interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / physiology*
  • Antigens, Differentiation, B-Lymphocyte / physiology*
  • B-Lymphocyte Subsets / cytology*
  • CD4-Positive T-Lymphocytes / cytology*
  • CD40 Antigens
  • CD40 Ligand
  • Humans
  • Immunoglobulin D / immunology
  • Interleukin-2 / physiology*
  • Interleukins / pharmacology
  • Lymphocyte Activation* / drug effects
  • Lymphocyte Cooperation
  • Membrane Glycoproteins / physiology


  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • CD40 Antigens
  • Immunoglobulin D
  • Interleukin-2
  • Interleukins
  • Membrane Glycoproteins
  • CD40 Ligand