The catalytic properties of the reverse transcriptase of the lentivirus equine infectious anemia virus

Eur J Biochem. 1994 Feb 1;219(3):977-83. doi: 10.1111/j.1432-1033.1994.tb18580.x.

Abstract

The reverse transcriptase (RT) of equine infectious anemia virus (EIAV) shares sequence similarity with the RTs of other lentiviruses, particularly with the RTs of human immunodeficiency viruses types 1 and 2 (HIV-1 and HIV-2, respectively), the causative agents of acquired immunodeficiency syndrome (AIDS). There is a 41-42% sequence identity between EIAV RT and both HIV RTs (which have 61% sequence identity to each other). We have compared the enzymic properties of EIAV RT with those of HIV-1 RT. Several aspects of the activities of EIAV RT differ from the corresponding activities of HIV-1 RT. There are significant differences in the inhibition of the DNA polymerase activities by the deoxynucleoside triphosphate analogs, 3'-azido-2,3'-dideoxythymidine triphosphate, dideoxyTTP and dideoxyGTP and by the nonnucleoside inhibitor, tetrahydroimidazo[4,5,1-jk-1,4]benzodiazepin-2-(1H)-one and thione; in the dependence of DNA polymerase and RNase H activities on pH; in the inhibition of the DNA polymerase activities by the thiol-specific reagent N-ethylmaleimide; in the specific DNA polymerase activity; in the inhibition of the ribonuclease H activity by the zinc chelator orthophenanthroline. However, there are several cases in which EIAV RT and HIV-1 RT are more similar than was previously found for HIV-1 RT and HIV-2 RT. These include the Km values for the DNA polymerase activities, the heat stability of the DNA polymerase functions and the specific activity of the RNase H function.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antiviral Agents / pharmacology
  • Benzodiazepines / pharmacology
  • Catalysis
  • DNA-Directed DNA Polymerase / metabolism
  • Enzyme Stability
  • Ethylmaleimide / pharmacology
  • HIV Reverse Transcriptase
  • Hot Temperature
  • Hydrogen-Ion Concentration
  • Imidazoles / pharmacology
  • Infectious Anemia Virus, Equine / enzymology*
  • Phenanthrolines / pharmacology
  • RNA-Directed DNA Polymerase / metabolism*
  • Recombinant Proteins / metabolism
  • Reverse Transcriptase Inhibitors
  • Ribonuclease H / metabolism

Substances

  • Antiviral Agents
  • Imidazoles
  • Phenanthrolines
  • Recombinant Proteins
  • Reverse Transcriptase Inhibitors
  • Benzodiazepines
  • R 82913
  • HIV Reverse Transcriptase
  • RNA-Directed DNA Polymerase
  • DNA-Directed DNA Polymerase
  • Ribonuclease H
  • Ethylmaleimide
  • 1,10-phenanthroline