Abstract
As a first approach to study the cellular events involved in myotonic dystrophy, we have produced a polyclonal antibody against a peptide sequence of the predicted gene product. This antibody specifically recognizes a 54 kDa protein in human skeletal muscle. This protein phosphorylates a co-polymer Glu/Tyr but not Myelin Basic Protein. This indicates that the myotonin-protein kinase has a tyrosine kinase activity in human skeletal muscle. This is the first demonstration of the kinase activity of the myotonin-protein kinase.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Cloning, Molecular
-
Humans
-
Immunologic Techniques
-
Molecular Sequence Data
-
Muscle Proteins / metabolism
-
Myotonic Dystrophy / enzymology
-
Myotonin-Protein Kinase
-
Peptides / chemistry
-
Phosphotyrosine
-
Protein Kinases / metabolism*
-
Protein Serine-Threonine Kinases*
-
Protein-Tyrosine Kinases / metabolism*
-
Recombinant Fusion Proteins
-
Tyrosine / analogs & derivatives*
-
Tyrosine / metabolism
Substances
-
DMPK protein, human
-
Muscle Proteins
-
Peptides
-
Recombinant Fusion Proteins
-
Phosphotyrosine
-
Tyrosine
-
Protein Kinases
-
Protein-Tyrosine Kinases
-
Myotonin-Protein Kinase
-
Protein Serine-Threonine Kinases