Cloning of FRK, a novel human intracellular SRC-like tyrosine kinase-encoding gene

Gene. 1994 Jan 28;138(1-2):247-51. doi: 10.1016/0378-1119(94)90817-6.

Abstract

We report the cloning of a novel tyrosine kinase (TyK)-encoding gene (TYK) from the human hepatoma cell line Hep3B. Using the polymerase chain reaction (PCR) and oligodeoxyribonucleotide primers based on conserved TYK motifs, a 180-bp fragment was cloned and used to obtain full-length cDNA clones of 2.9 kb, with an open reading frame of 505 amino acids (aa). Restricted expression was detected by Northern blotting or reverse-transcribed PCR in a broad range of cell lines. The predicted aa sequence contains characteristic TyK motifs without a transmembrane region, suggesting an intracellular localization. There was 49% aa sequence identity with human FYN product and 47% with human SRC product; however, several structural differences distinguish this clone from other SRC subfamily members. This clone, FYN-related kinase or FRK, is a novel member of the intracellular TYK gene family.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Carcinoma, Hepatocellular
  • Cell Line
  • Cloning, Molecular
  • Conserved Sequence
  • DNA Primers
  • DNA, Complementary / metabolism
  • Genes, src*
  • Humans
  • Liver Neoplasms
  • Molecular Sequence Data
  • Multigene Family*
  • Neoplasm Proteins*
  • Poly A / analysis
  • Polymerase Chain Reaction
  • Protein-Tyrosine Kinases / biosynthesis
  • Protein-Tyrosine Kinases / genetics*
  • RNA / analysis
  • RNA, Messenger
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured

Substances

  • DNA Primers
  • DNA, Complementary
  • Neoplasm Proteins
  • RNA, Messenger
  • Poly A
  • RNA
  • Protein-Tyrosine Kinases
  • FRK protein, human

Associated data

  • GENBANK/U00803