Pulmonary microvascular injury after intestinal ischemia-reperfusion: role of P-selectin

J Appl Physiol (1985). 1993 Dec;75(6):2529-34. doi: 10.1152/jappl.1993.75.6.2529.


The objective of this study was to determine whether pulmonary endothelial expression of the adhesive glycoprotein P-selectin contributes to the lung injury and leukostasis observed after intestinal ischemia-reperfusion (I/R). The pulmonary capillary filtration coefficient and lung myeloperoxidase activity were determined in rat lungs isolated after 120 min of superior mesenteric artery occlusion and 90 min of reperfusion. Intestinal I/R resulted in a marked increase in the pulmonary capillary filtration coefficient compared with control and sham-operated rats. The increase in pulmonary microvascular permeability elicited by intestinal I/R was effectively prevented by pretreatment with a P-selectin monoclonal antibody (MAb; MAb PB1.3) but was unaffected by a control MAb. The intestinal I/R-induced increase in pulmonary microvascular permeability was accompanied by a dramatic sequestration of granulocytes in the lung compared with control and sham-operated rats; however, neither the P-selectin nor the control MAbs affected this event. These results indicate that P-selectin contributes to the pulmonary microvascular dysfunction observed after intestinal I/R. The inhibition of intestinal I/R-induced lung injury by immunoneutralization of P-selectin appears to be unrelated to the accompanying lung leukosequestration.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Blood Pressure / physiology
  • Capillary Permeability / physiology*
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / physiology*
  • Intestines / blood supply*
  • Ischemia / physiopathology*
  • Leukocyte Count
  • Lung Diseases / physiopathology*
  • Male
  • Organ Size
  • P-Selectin
  • Peroxidase / metabolism
  • Platelet Membrane Glycoproteins / immunology
  • Platelet Membrane Glycoproteins / physiology*
  • Pulmonary Circulation / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / physiopathology*


  • Antibodies, Monoclonal
  • Cell Adhesion Molecules
  • P-Selectin
  • Platelet Membrane Glycoproteins
  • Peroxidase