Local proliferation of fibroblast-like synoviocytes contributes to synovial hyperplasia. Results of proliferating cell nuclear antigen/cyclin, c-myc, and nucleolar organizer region staining

Arthritis Rheum. 1994 Feb;37(2):212-20. doi: 10.1002/art.1780370210.


Objective: To test the hypothesis that local proliferation contributes significantly to the hyperplasia of rheumatoid synovium.

Methods: Immunohistologic and chemical staining was used to identify 3 markers of cell proliferation: proliferating cell nuclear antigen, c-myc proto-oncogene, and nucleolar organizer regions. Synovium from 21 patients with rheumatoid arthritis, 34 with degenerative joint disease, and 7 with joint trauma was examined.

Results: All 3 markers indicated substantial, active proliferation of synovial lining cells in synovium with hyperplasia. Proliferating cells showed type I procollagen immunoreactivity but were negative for CD68, a monocyte/macrophage marker. Proliferation was greater in rheumatoid arthritis than in the other conditions evaluated.

Conclusion: In situ proliferation of fibroblast-like synoviocytes in the synovium lining contributes considerably to the increase in cell numbers in rheumatoid synovium.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Autoantigens / metabolism
  • Cell Division
  • Female
  • Fibroblasts / cytology*
  • Humans
  • Hyperplasia
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Nuclear Proteins / metabolism*
  • Nucleolus Organizer Region / ultrastructure*
  • Proliferating Cell Nuclear Antigen
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Staining and Labeling
  • Synovial Membrane / metabolism*
  • Synovial Membrane / pathology*


  • Autoantigens
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • Proto-Oncogene Proteins c-myc