Rapid detection and identification of Vibrio anguillarum by using a specific oligonucleotide probe complementary to 16S rRNA

Appl Environ Microbiol. 1994 Feb;60(2):732-7. doi: 10.1128/aem.60.2.732-737.1994.


Partial 16S rDNA from Vibrio collection type strains and recent isolates of Vibrio-related strains were sequenced and compared with previously published sequences. A 24-base DNA oligonucleotide (VaV3) was designed and used as a specific probe for detection and identification of Vibrio anguillarum. Its specificity was tested against collection type strains and environmental isolates and no cross-reaction was found. The probe detected 8 of the 10 V. anguillarum serovars. It was applied to screen different Vibrio-related strains isolated from marine hatcheries and fish farms. The detection limit in DNA-DNA slot blot hybridization was 150 pg.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • RNA, Bacterial / genetics
  • Sensitivity and Specificity
  • Species Specificity
  • Time Factors
  • Vibrio / genetics
  • Vibrio / isolation & purification*


  • DNA, Bacterial
  • DNA, Ribosomal
  • Oligonucleotide Probes
  • RNA, Bacterial