Fas-based lymphocyte-mediated cytotoxicity against syngeneic activated lymphocytes: a regulatory pathway?

Eur J Immunol. 1994 Apr;24(4):923-7. doi: 10.1002/eji.1830240421.

Abstract

To investigate the possibility that Fas-based immune regulation and Fas-based T cell-mediated cytotoxicity (F-CMC) are causally related, we explored the latter in activated lymphocyte populations. These were shown to contain effector cells exerting cytotoxicity via an F-CMC mechanism which could be differentially triggered by PMA and ionomycin. F-CMC operated in trans, requiring an lpr (Fas) product on target cells and a gld product on effector cells. Activated lymphocyte populations were also shown to contain F-CMC target cells. Activated lymphocyte populations thus contained both effector and target F-CMC cells, which could lyse each other. Fas-based cytotoxicity can thus lead to the lysis of syngeneic activated lymphocytes, consistent with the possibility of its participation in the down-regulation of immune responses, and more generally offering a model of socially controlled, direct membrane-mediated cell death.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / physiology*
  • Cell Death
  • Cytotoxicity, Immunologic*
  • Ionomycin / pharmacology
  • Lymphocyte Activation*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • T-Lymphocytes / immunology*
  • Tetradecanoylphorbol Acetate / pharmacology
  • fas Receptor

Substances

  • Antigens, Surface
  • fas Receptor
  • Ionomycin
  • Tetradecanoylphorbol Acetate