Occurrence of antibodies against chlamydial lipopolysaccharide in human sera as measured by ELISA using an artificial glycoconjugate antigen

FEMS Immunol Med Microbiol. 1994 Jan;8(1):27-41. doi: 10.1111/j.1574-695X.1994.tb00422.x.

Abstract

An artificial glycoconjugate containing, as a ligand, the deacylated carbohydrate backbone of a recombinant Chlamydia-specific lipopolysaccharide was used as a solid-phase antigen in ELISA to measure antibodies against chlamydial LPS. The specificity and reproducibility of the assay was shown by using a panel of prototype monoclonal antibodies representing the spectrum of antibodies also occurring in patient sera. These mAbs recognized Chlamydia-specific epitopes [alpha 2-->8-linked disaccharide of 3-deoxy-D-manno-octulosonic acid (Kdo) or the trisaccharide alpha Kdo-(2-->8)-alpha Kdo-(2-->4)-alpha Kdo] or those shared between chlamydial and Re-type LPS (alpha Kdo, alpha 2-->4-linked Kdo disaccharide). The assay was used to measure IgG, IgA and IgM antibodies against chlamydial LPS in patients with genital or respiratory tract infections. In comparison to the results obtained with sera from blood donors, it became evident that both types of infection result in significant changes in the profile of LPS antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / blood*
  • Antibodies, Monoclonal / immunology
  • Antigens, Bacterial / immunology*
  • Blood Donors
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chlamydia / immunology*
  • Chlamydia Infections / blood
  • Chlamydia Infections / immunology
  • Chlamydia Infections / prevention & control
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay*
  • Epitopes / chemistry
  • Epitopes / immunology
  • Female
  • Glycoconjugates / chemical synthesis
  • Glycoconjugates / immunology*
  • Humans
  • Lipopolysaccharides / chemistry
  • Lipopolysaccharides / immunology*
  • Male
  • Mass Screening
  • Molecular Sequence Data
  • Recombinant Proteins / immunology
  • Reproducibility of Results
  • Respiratory Tract Infections / blood
  • Respiratory Tract Infections / immunology
  • Respiratory Tract Infections / microbiology
  • Sensitivity and Specificity
  • Species Specificity
  • Urethritis / blood
  • Urethritis / immunology
  • Urethritis / microbiology
  • Uterine Cervicitis / blood
  • Uterine Cervicitis / immunology
  • Uterine Cervicitis / microbiology

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Epitopes
  • Glycoconjugates
  • Lipopolysaccharides
  • Re lipopolysaccharide
  • Recombinant Proteins