Impaired neurite outgrowth of src-minus cerebellar neurons on the cell adhesion molecule L1

Neuron. 1994 Apr;12(4):873-84. doi: 10.1016/0896-6273(94)90339-5.


The nonreceptor tyrosine protein kinases pp60c-src, p59fyn, and pp62c-yes are localized in growth cones of developing neurons, but their function is undefined. To determine whether these tyrosine kinases were capable of regulating substrate-dependent axon growth, cultures of cerebellar neurons from wild-type, src-, fyn-, and yes- mice were analyzed for neurite outgrowth on the neural cell adhesion molecule L1 or the extracellular matrix protein laminin. The rate of neurite extension on L1 was reduced in src-, but not in fyn- or yes- neurons. Neurite extension on laminin was unaltered in src-, fyn-, or yes- neurons, indicating that pp60c-src, p59fyn, or pp62c-yes is not likely to participate in integrin-dependent axon growth. These results demonstrate that pp60c-src is a component of the intracellular signaling pathway in L1-mediated axonal growth and suggest that Src-related nonreceptor tyrosine kinases may have distinct, nonredundant functions in the nervous system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Adhesion Molecules, Neuronal / physiology*
  • Cells, Cultured
  • Cerebellum / cytology*
  • Immunoblotting
  • Laminin / physiology
  • Leukocyte L1 Antigen Complex
  • Mice
  • Mice, Inbred C57BL
  • Mutation
  • Neurites / physiology*
  • Neurons / physiology*
  • Phosphorylation
  • Phosphotyrosine
  • Protein-Tyrosine Kinases / physiology*
  • Proto-Oncogene Proteins pp60(c-src) / physiology*
  • Tyrosine / analogs & derivatives
  • Tyrosine / metabolism


  • Cell Adhesion Molecules, Neuronal
  • Laminin
  • Leukocyte L1 Antigen Complex
  • Phosphotyrosine
  • Tyrosine
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins pp60(c-src)