Three-dimensional solution structure of the extracellular region of the complement regulatory protein CD59, a new cell-surface protein domain related to snake venom neurotoxins

Biochemistry. 1994 Apr 19;33(15):4471-82.


The cell surface antigen CD59 is an inhibitor of complement-mediated lysis and a member of the Ly6 superfamily (Ly6SF) of cysteine-rich cell-surface molecules whose sequences are related to those of snake venom neurotoxins. The three-dimensional solution structure of a recombinant form of the extracellular region of the molecule (residues 1-70 of the mature protein; sCD59) has been solved by 2D NMR methods. sCD59 is a relatively flat, disk-shaped molecule consisting of a two-standed beta-sheet finger loosely packed against a protein core formed by a three-stranded beta-sheet and a short helix. Structure calculations allowed an unambiguous assignment of the disulfide-bonded cysteine pairs as 3-26, 6-13, 19-39, 45-63, and 64-69. The topology of sCD59 is similar to that of the snake venom neurotoxins and consistent with an evolutionary relationship existing between the Ly6SF and the neurotoxins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, CD / chemistry*
  • CD59 Antigens
  • Crystallization
  • Disulfides / chemistry
  • Electrochemistry
  • Extracellular Space / chemistry
  • Hydrogen Bonding
  • Magnetic Resonance Spectroscopy
  • Membrane Glycoproteins / chemistry*
  • Models, Molecular
  • Molecular Sequence Data
  • Molecular Structure
  • Neurotoxins / chemistry*
  • Protein Structure, Secondary
  • Recombinant Proteins / chemistry
  • Snake Venoms / chemistry*
  • Solutions


  • Antigens, CD
  • CD59 Antigens
  • Disulfides
  • Membrane Glycoproteins
  • Neurotoxins
  • Recombinant Proteins
  • Snake Venoms
  • Solutions

Associated data

  • PIR/A39743
  • PIR/A46528
  • PIR/P01391
  • PIR/P01435
  • PIR/P01471
  • PIR/P05533
  • PIR/P13987
  • SWISSPROT/A39743
  • SWISSPROT/A46528
  • SWISSPROT/P01391
  • SWISSPROT/P01435
  • SWISSPROT/P01471
  • SWISSPROT/P05533
  • SWISSPROT/P13987