Detection of insulin-like growth factor binding proteins (IGFBPs) by ligand blotting in breast cancer tissues

Cancer Lett. 1994 Feb 28;77(1):25-32. doi: 10.1016/0304-3835(94)90343-3.


Eighty breast cancer specimens were examined for insulin-like growth factor binding protein (IGFBP) expression by ligand blotting. Five distinct IGFBP species were found: a doublet at 48 and 44 kDa was IGFBP-3, the 34-kDa band was IGFBP-2, and a band at 24 kDa was IGFBP-4. A 32-kDa band was compatible with the migration position reported for IGFBP-5. IGFBP-3 was inversely correlated with ER expression, while IGFBP-4 was positively correlated with both ER and PgR. IGFBP-4 was also inversely correlated with S-phase fraction. Thus, IGFBP expression correlates with other parameters of breast cancer biology and may play a role in regulating tumor growth.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Breast Neoplasms / chemistry*
  • Breast Neoplasms / pathology
  • Carrier Proteins / analysis*
  • Cell Division
  • Female
  • Humans
  • Insulin-Like Growth Factor Binding Proteins
  • Receptors, Estrogen / analysis
  • Receptors, Progesterone / analysis
  • Somatomedins / metabolism*


  • Carrier Proteins
  • Insulin-Like Growth Factor Binding Proteins
  • Receptors, Estrogen
  • Receptors, Progesterone
  • Somatomedins