Assembly and regulation of NADPH oxidase and nitric oxide synthase

Curr Opin Immunol. 1994 Feb;6(1):131-9. doi: 10.1016/0952-7915(94)90044-2.


Research over the past year has revealed several interesting advances in the biosynthesis of the superoxide anion and nitric oxide. Highlights include the demonstration that the G protein Rac 2 is required for NADPH oxidase activation, the finding that nitric oxide is a feedback inhibitor of nitric oxide synthase isoforms, and the discovery that the continuous catalytic activity of the immune/inflammatory nitric oxide synthase is due to strong calmodulin binding, which is independent of elevated calcium levels. Interferon-gamma primes neutrophils and macrophages for both O2- and nitric oxide synthesis. However, NADPH oxidase and immune/inflammatory nitric oxide synthase are differentially regulated such that their activities are not simultaneously induced.

Publication types

  • Review

MeSH terms

  • Amino Acid Oxidoreductases / biosynthesis*
  • Amino Acid Oxidoreductases / metabolism
  • Animals
  • Enzyme Activation
  • Humans
  • Isoenzymes / metabolism
  • NADH, NADPH Oxidoreductases / biosynthesis*
  • NADH, NADPH Oxidoreductases / metabolism
  • NADPH Oxidases
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase
  • Phagocytes / enzymology
  • Superoxides / metabolism


  • Isoenzymes
  • Superoxides
  • Nitric Oxide
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases
  • NADH, NADPH Oxidoreductases
  • NADPH Oxidases