A monoclonal antibody inhibits adhesion to fibronectin and vitronectin of a colon carcinoma cell line and recognizes the integrins alpha v beta 3, alpha v beta 5, and alpha v beta 6

Cancer Res. 1994 Apr 15;54(8):2102-7.


Using whole viable human colon carcinoma HT29 cells as immunogen, we produced a monoclonal antibody (mAb) termed 69-6-5. The antibody was functionally selected on its anti-cell-spreading activity. By immunoprecipitation of surface radiolabeled cell lysates from HT29-D4 cells (an HT29 cell clone), mAb 69-6-5 recognized a molecular complex resembling integrin heterodimers. Sequential immunodepletions with mAb to the integrin alpha v subunit demonstrated that this complex was composed of alpha v-containing integrins. Accordingly, mAb 69-6-5 reacted with integrin alpha v beta 3 immunopurified from melanoma cells and integrins alpha v beta 5 and alpha v beta 6 immunopurified from pancreatic carcinoma cells. In cell adhesion assays, the 69-6-5 mAb was able to inhibit strongly in a dose-dependent manner arginine-glycine-aspartic acid-mediated adhesion of HT29-D4 cells to vitronectin, fibronectin, or ProNectin F but not to laminin or collagen. Immunoprecipitations with beta chain-specific antisera indicated that these cells express integrins alpha v beta 5 (receptor for vitronectin) and alpha v beta 6 (receptor for fibronectin) but neither alpha v beta 1 nor alpha v beta 3. In summary, these results indicated that mAb 69-6-5 reacts with several alpha v integrins and that it can effectively interfere with the adhesive functions of at least alpha v beta 5 and alpha v beta 6, which represent the major receptors on HT29-D4 cells responsible for their adhesion on vitronectin and fibronectin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / pharmacology*
  • Antigens, Neoplasm*
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology*
  • Cell Line
  • Cell Movement
  • Colonic Neoplasms
  • Cross Reactions
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes / chemistry
  • Epitopes / immunology
  • Fibronectins*
  • Glycoproteins*
  • Humans
  • Integrins / analysis
  • Integrins / immunology
  • Integrins / physiology*
  • Molecular Sequence Data
  • Molecular Weight
  • Receptors, Cytoadhesin / immunology
  • Receptors, Cytoadhesin / physiology
  • Receptors, Vitronectin
  • Tumor Cells, Cultured
  • Vitronectin


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Epitopes
  • Fibronectins
  • Glycoproteins
  • Integrins
  • Receptors, Cytoadhesin
  • Receptors, Vitronectin
  • Vitronectin
  • integrin alphaVbeta5
  • integrin alphavbeta6