Molecular Cloning and Expression of a Novel Human Gene That Is Highly Homologous to Human FK506-binding Protein 12kDa (hFKBP-12) and Characterization of Two Alternatively Spliced Transcripts

Biochem Biophys Res Commun. 1994 Apr 29;200(2):836-43. doi: 10.1006/bbrc.1994.1527.

Abstract

We isolated a novel gene encoding a protein highly homologous to human FK506-binding protein 12kDa (hFKBP-12) from a human fetal brain cDNA library and determined the full-length cDNA sequence. The cDNA clone contained the open reading frame of 324 nucleotides encoding 108 amino acid and revealed 76% identity in DNA sequence and 88% identity in predicted amino acid sequence with hFKBP-12. The DNA and amino-acid sequence of this gene, designated OTK4, also had homology with other FKBPs in species ranging from humans to prokaryotes. Recombinant protein, produced in E.coli transformed by a pGEX2T expression vector containing the OTK4 cDNA and purified, showed peptidyl-prolyl cis-trans isomerase activity like other FKBP proteins. An alternatively spliced form of the transcript found in the cDNA library contained a 45-bp insertion which included a stop codon. Although the biological function of the truncated version of OTK4 is unknown, both transcripts were ubiquitously expressed in human tissues examined by the reverse-transcriptase PCR (RT-PCR) method.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Isomerases / genetics
  • Amino Acid Isomerases / metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Gene Expression
  • Heat-Shock Proteins / chemistry
  • Heat-Shock Proteins / genetics*
  • Heat-Shock Proteins / metabolism
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Peptidylprolyl Isomerase
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Tacrolimus / metabolism*
  • Tacrolimus Binding Proteins

Substances

  • Carrier Proteins
  • DNA Primers
  • DNA, Complementary
  • Heat-Shock Proteins
  • Recombinant Fusion Proteins
  • Amino Acid Isomerases
  • Tacrolimus Binding Proteins
  • Peptidylprolyl Isomerase
  • Tacrolimus

Associated data

  • GENBANK/D38037