Cloning and expression of a cDNA for the human prostacyclin receptor

FEBS Lett. 1994 May 9;344(1):74-8. doi: 10.1016/0014-5793(94)00355-6.


A functional cDNA for the human prostacyclin receptor was isolated from a cDNA library of CMK cells, a human megakaryocytic leukaemia cell line. The cDNA encodes a protein consisting of 386 amino acid residues with seven putative transmembrane domains and a deduced molecular weight of 40,956. [3H]Iloprost specifically bound to the membrane of CHO cells stably expressing the cDNA with a Kd of 3.3 nM. This binding was displaced by unlabelled prostanoids in the order of iloprost = cicaprost >> carbacyclin > prostaglandin E1 (PGE1) > STA2. PGE2, PGD2 and PGF 2 alpha did not inhibit it. Iloprost in a concentration-dependent manner increased the cAMP level and generated inositol trisphosphate in these cells, indicating that this human receptor can couple to multiple signal transduction pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding, Competitive
  • CHO Cells
  • Cloning, Molecular*
  • Cricetinae
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics*
  • Gene Expression*
  • Gene Transfer Techniques
  • Humans
  • Iloprost / metabolism
  • Mice
  • Molecular Sequence Data
  • Receptors, Epoprostenol
  • Receptors, Prostaglandin / chemistry
  • Receptors, Prostaglandin / genetics*
  • Recombinant Proteins / metabolism
  • Thrombocythemia, Essential
  • Tumor Cells, Cultured


  • DNA, Complementary
  • Receptors, Epoprostenol
  • Receptors, Prostaglandin
  • Recombinant Proteins
  • Iloprost

Associated data

  • GENBANK/D25418