VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells

J Virol. 1994 Jun;68(6):3453-8. doi: 10.1128/JVI.68.6.3453-3458.1994.

Abstract

Murine VCAM-1 has been identified as a receptor for the D variant of encephalomyocarditis (EMC-D) virus on vascular endothelial cells from the heart. Monoclonal antibodies to VCAM-1 inhibited infection and lysis of endothelial cells with EMC-D virus. CHO cells transfected with the VCAM-1 gene were susceptible to EMC-D virus lysis, while control CHO cells transfected with the ELAM-1 gene were resistant. Similarly, 35S-labeled EMC-D virus bound to CHO-VCAM cells, and binding was inhibited with anti-VCAM-1 antibodies. Little or no radiolabeled virus bound to CHO-ELAM-1 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • CHO Cells
  • Cardiovirus Infections / etiology
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / physiology*
  • Cricetinae
  • Encephalomyocarditis virus / pathogenicity
  • Encephalomyocarditis virus / physiology*
  • Endothelium, Vascular / microbiology*
  • Endothelium, Vascular / physiology
  • In Vitro Techniques
  • Mice
  • Mice, Inbred BALB C
  • Receptors, Virus / antagonists & inhibitors
  • Receptors, Virus / immunology
  • Receptors, Virus / physiology*
  • Transfection
  • Vascular Cell Adhesion Molecule-1

Substances

  • Antibodies, Monoclonal
  • Cell Adhesion Molecules
  • Receptors, Virus
  • Vascular Cell Adhesion Molecule-1