Abstract
Murine VCAM-1 has been identified as a receptor for the D variant of encephalomyocarditis (EMC-D) virus on vascular endothelial cells from the heart. Monoclonal antibodies to VCAM-1 inhibited infection and lysis of endothelial cells with EMC-D virus. CHO cells transfected with the VCAM-1 gene were susceptible to EMC-D virus lysis, while control CHO cells transfected with the ELAM-1 gene were resistant. Similarly, 35S-labeled EMC-D virus bound to CHO-VCAM cells, and binding was inhibited with anti-VCAM-1 antibodies. Little or no radiolabeled virus bound to CHO-ELAM-1 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal
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CHO Cells
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Cardiovirus Infections / etiology
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / physiology*
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Cricetinae
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Encephalomyocarditis virus / pathogenicity
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Encephalomyocarditis virus / physiology*
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Endothelium, Vascular / microbiology*
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Endothelium, Vascular / physiology
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In Vitro Techniques
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Mice
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Mice, Inbred BALB C
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Receptors, Virus / antagonists & inhibitors
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Receptors, Virus / immunology
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Receptors, Virus / physiology*
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Transfection
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Vascular Cell Adhesion Molecule-1
Substances
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Antibodies, Monoclonal
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Cell Adhesion Molecules
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Receptors, Virus
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Vascular Cell Adhesion Molecule-1