Radioimmunoassay versus flow cytometric assay to quantify LPS-binding protein (LBP) concentrations in human plasma

J Immunol Methods. 1994 May 16;171(2):169-76. doi: 10.1016/0022-1759(94)90037-x.


LPS-binding protein (LBP) is an acute phase protein present in plasma, that has been shown to play a major role in sensitizing monocytes to LPS. We describe here two assays to quantify LBP in plasma. The first assay made use of monophosphoryl lipid A to capture LBP in human plasma, and LBP was detected by radiolabeled anti-LBP IgG. The second assay measured LBP by flow cytometry, using LBPs ability to present LPS to the CD14 receptor present on monocytes. Both assays had a similar level of sensitivity, that allowed the quantification of LBP in human plasma.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute-Phase Proteins / analysis*
  • Antibody Specificity
  • Carrier Proteins / blood*
  • Flow Cytometry
  • Humans
  • Immunoglobulin G
  • Lipid A / analogs & derivatives
  • Membrane Glycoproteins*
  • Radioimmunoassay
  • Sensitivity and Specificity


  • Acute-Phase Proteins
  • Carrier Proteins
  • Immunoglobulin G
  • Lipid A
  • Membrane Glycoproteins
  • lipopolysaccharide-binding protein
  • monophosphoryl lipid A