beta-Amyloid related peptides exert differential effects on [3H]MK-801 binding to rat cortical membranes

Neuroreport. 1994 Jan 12;5(4):405-8. doi: 10.1097/00001756-199401120-00008.

Abstract

The effects of beta-amyloid protein 1-40 (beta AP 1-40), substance P (SP), and the amidated and carboxylic acid C-terminated forms of the SP homologous beta AP fragment 25-35 (beta AP 25-35-NH2 and beta AP 25-35-COOH) were studied on [3H]MK-801 binding to the rat brain NMDA receptor cation channel. All peptides gave dose-dependent enhancements of [3H]MK-801 binding stimulated by low glycine. beta AP 25-35-COOH, but not beta AP 25-35-NH2 produced an inhibition of [3H]MK-801 binding stimulated by high glycine in the presence of either low or high glutamate. Low glutamate-stimulated [3H]MK-801 binding was also inhibited by SP but not by beta AP 1-40. It is concluded that beta AP related peptides exert differential effects on the NMDA receptor complex at the glycine and possibly also the glutamate recognition sites.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides / pharmacology*
  • Animals
  • Cerebral Cortex / drug effects*
  • Cerebral Cortex / metabolism
  • Dizocilpine Maleate / metabolism*
  • Male
  • Membranes / drug effects
  • Membranes / metabolism
  • Nerve Tissue Proteins / pharmacology*
  • Peptide Fragments / pharmacology*
  • Radioligand Assay
  • Rats
  • Rats, Sprague-Dawley
  • Substance P / pharmacology*
  • Tritium

Substances

  • Amyloid beta-Peptides
  • Nerve Tissue Proteins
  • Peptide Fragments
  • Tritium
  • Substance P
  • Dizocilpine Maleate