Abstract
The Mip ('macrophage infectivity potentiator') protein of Legionella pneumophila has been shown to be an essential virulence factor, exhibiting peptidyl-prolyl cis/trans isomerase (PPIase) activity that can be inhibited by the immunosuppressant FK506. The cloning and sequencing of mip genes from three different L. pneumophila strains revealed a single amino acid substitution which did not affect the isomerase property of the enzyme. Mip proteins isolated from two wild-type L. pneumophila strains and from two corresponding Escherichia coli K-12 recombinant clones derived from these strains exhibited identical enzymatic properties and the precursor proteins are processed at identical cleavage sites. The mature Mip proteins exist in an oligomeric form. Site-directed mutagenesis demonstrated that a substitution of an Asp residue at position 142 by a Leu residue affects PPIase activity of Mip.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Isomerases / metabolism
-
Amino Acid Sequence
-
Bacterial Proteins* / analysis
-
Bacterial Proteins* / chemistry
-
Bacterial Proteins* / genetics
-
Bacterial Proteins* / isolation & purification
-
Bacterial Proteins* / metabolism
-
Base Sequence
-
Carrier Proteins / metabolism
-
Cloning, Molecular
-
DNA, Bacterial / analysis
-
Genes, Bacterial / genetics*
-
Immunophilins*
-
Kinetics
-
Legionella pneumophila / chemistry*
-
Legionella pneumophila / genetics*
-
Legionella pneumophila / pathogenicity
-
Membrane Proteins* / analysis
-
Membrane Proteins* / chemistry
-
Membrane Proteins* / genetics
-
Membrane Proteins* / isolation & purification
-
Membrane Proteins* / metabolism
-
Molecular Sequence Data
-
Molecular Weight
-
Mutagenesis, Site-Directed
-
Peptidylprolyl Isomerase
-
Protein Conformation
-
Sequence Analysis
-
Sequence Analysis, DNA
-
Tacrolimus / metabolism
-
Virulence
Substances
-
Bacterial Proteins
-
Carrier Proteins
-
DNA, Bacterial
-
Membrane Proteins
-
Amino Acid Isomerases
-
Immunophilins
-
Mip protein, Legionella pneumophila
-
Peptidylprolyl Isomerase
-
Tacrolimus