Decay-accelerating factor (CD55), a glycosylphosphatidylinositol-anchored complement regulatory protein, is a receptor for several echoviruses

Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6245-8. doi: 10.1073/pnas.91.13.6245.


Echoviruses are human pathogens belonging to the picornavirus family. Decay-accelerating factor (DAF) is a glycosylphosphatidylinositol (GPI)-anchored surface protein that protects cells from lysis by autologous complement. Anti-DAF monoclonal antibodies prevented echovirus 7 attachment to susceptible cells and protected cells from infection. HeLa cells specifically lost the capacity to bind echovirus 7 when treated with phosphatidylinositol-specific phospholipase C, an enzyme that releases GPI-anchored proteins from the cell surface, indicating that the virus receptor, like DAF, is a GPI-anchored protein. Although Chinese hamster ovary cells do not bind echovirus 7, transfectants expressing human DAF bound virus efficiently, and binding was prevented by pretreatment with an anti-DAF monoclonal antibody. Anti-DAF antibodies prevented infection by at least six echovirus serotypes. These results indicate that DAF is the receptor mediating attachment and infection by several echoviruses.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / physiology*
  • CD55 Antigens
  • Complement Inactivator Proteins / physiology*
  • Enterovirus B, Human / physiology*
  • Glycosylphosphatidylinositols / physiology
  • HeLa Cells
  • Humans
  • Membrane Glycoproteins / physiology*
  • Mice
  • Mice, Inbred BALB C / immunology
  • Phosphatidylinositol Diacylglycerol-Lyase
  • Phosphoinositide Phospholipase C
  • Phosphoric Diester Hydrolases / pharmacology
  • Receptors, Virus / drug effects
  • Receptors, Virus / physiology*


  • Antibodies, Monoclonal
  • Antigens, CD
  • CD55 Antigens
  • Complement Inactivator Proteins
  • Glycosylphosphatidylinositols
  • Membrane Glycoproteins
  • Receptors, Virus
  • Phosphoric Diester Hydrolases
  • Phosphoinositide Phospholipase C
  • Phosphatidylinositol Diacylglycerol-Lyase