Antibodies specific for the neuronal form of the Src protein elicited by an antigenized antibody

DNA Cell Biol. 1994 Jun;13(6):585-91. doi: 10.1089/dna.1994.13.585.

Abstract

To elicit antibodies directed specifically against the neuron-specific form of the c-src gene product, pp60c-src(+), we used an antigenized antibody comprising a decamer containing the amino acid sequence specific to pp60c-src(+) inserted into the third hypervariable loop of the heavy (H)-chain variable (V)-region. This was used to raise anti-idiotype antibodies reacting with the peptide epitope in rabbits. The antisera reacted with pp60c-src(+), as judged by immune blotting, immunoprecipitation, immune complex kinase assay, and indirect immunofluorescence staining, but did not react with the fibroblast form of the c-src gene product, pp60c-src. Antigenized antibody is a useful approach for producing antibodies able to distinguish between isoforms of the same gene product and specific for the neuronal form of the Src protein.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Antibodies / genetics
  • Antibodies / immunology*
  • Antibodies, Anti-Idiotypic / immunology
  • Antibody Specificity
  • Base Sequence
  • Cell Line
  • DNA
  • Epitopes
  • Female
  • Mice
  • Molecular Sequence Data
  • Neurons / immunology*
  • Proto-Oncogene Proteins pp60(c-src) / genetics
  • Proto-Oncogene Proteins pp60(c-src) / immunology*
  • Rabbits
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology

Substances

  • Antibodies
  • Antibodies, Anti-Idiotypic
  • Epitopes
  • Recombinant Proteins
  • DNA
  • Proto-Oncogene Proteins pp60(c-src)