Human astrocytomas and glioblastomas express monocyte chemoattractant protein-1 (MCP-1) in vivo and in vitro

Int J Cancer. 1994 Jul 15;58(2):240-7. doi: 10.1002/ijc.2910580216.


Expression of the monocyte chemoattractant protein-1 (MCP-1) was examined in human central nervous system tumours (glioblastomas and astrocytomas) and normal human brain. Northern blot analysis demonstrated constitutive expression of MCP-1 mRNA in 6 of 12 glioblastoma cell lines. Expression could be stimulated by interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in all cell lines tested. Immunoprecipitation demonstrated secretion of both isoforms, MCP-1 alpha and -beta, of the MCP-1 protein. Reverse-transcription polymerase chain reaction and Northern blot analysis on tissues demonstrated MCP-1 mRNA expression in 17 of 17 glioblastomas, 3 of 6 anaplastic astrocytomas and 6 of 6 low-grade astrocytomas, as well as in fetal brain but not in normal adult brain. In situ hybridization on 2 glioblastomas and 1 low-grade astrocytoma indicates that neoplastic astrocytes and endothelial cells express MCP-1 mRNA in vivo. Moreover, tumour cyst fluids of glioblastomas and astrocytomas were able to induce monocyte chemoattraction in an in vitro assay. This chemotactic activity was specifically neutralized by anti-MCP-1 antibodies in 9 of 10 samples, further demonstrating the production of bioactive MCP-1 in vivo and supporting an important role for this factor in the infiltration of monocytes/macrophages into tumour tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Astrocytoma / genetics
  • Astrocytoma / metabolism*
  • Base Sequence
  • Blotting, Northern
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism*
  • Chemokine CCL2
  • Chemotactic Factors / biosynthesis*
  • Chemotactic Factors / genetics
  • Chemotactic Factors / metabolism
  • Cytokines / biosynthesis
  • Cytokines / genetics
  • Cytokines / metabolism
  • Gene Expression
  • Glioblastoma / genetics
  • Glioblastoma / metabolism*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA-Directed DNA Polymerase
  • Tumor Cells, Cultured


  • Chemokine CCL2
  • Chemotactic Factors
  • Cytokines
  • RNA, Messenger
  • RNA-Directed DNA Polymerase