The CD28/B7 pathway costimulates the response of primary murine T cells to superantigens as well as to conventional antigens

Cell Immunol. 1994 Aug;157(1):306-12. doi: 10.1006/cimm.1994.1225.

Abstract

While the CD28/B7 pathway has been shown to play an important costimulatory role in the response of T cells to conventional antigens, its role in superantigen-mediated T cell activation has not been as clear. In this report, we used CD4+ T cells from ovalbumin-specific alpha beta-TCR-transgenic mice (DO11.10) to compare the ability of this pathway to costimulate the response of primary T cells to conventional antigens and superantigens. We show that either the addition of anti-CD28 monoclonal antibody or presentation with a B7 transfected cell line enhances the proliferative and interleukin-2 secretion response of DO11.10 CD4+ cells to both the conventional antigen, ovalbumin peptide (OVA 323-339), and the superantigen SEB. This implies that CD28-mediated costimulation plays a role in the response of murine T cells to superantigens as well as to conventional antigens.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens / immunology*
  • B7-1 Antigen / physiology*
  • CD28 Antigens / physiology*
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Line
  • Epitopes / immunology
  • Flow Cytometry
  • Interleukin-2 / analysis
  • Lymphocyte Activation
  • Mice
  • Mice, Transgenic
  • Ovalbumin / immunology
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Spleen / cytology
  • Superantigens / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens
  • B7-1 Antigen
  • CD28 Antigens
  • Epitopes
  • Interleukin-2
  • Receptors, Antigen, T-Cell, alpha-beta
  • Superantigens
  • Ovalbumin