Abstract
Neutralizing and non-neutralizing monoclonal antibodies against parapoxviruses (PPV) were generated by immunizing BALB/c-mice with gradient-purified PPV Orf D-1701 or purified envelopes. Epitope specificity studies identified three distinct epitopes localized in the virus envelope. These antigenic sites allowed a differentiation between orf and stomatitis papulosa viruses. For a rapid diagnosis of parapoxviruses transmission-electron microscopy, immunofluorescence- or immunoperoxidase-staining, antigen capture ELISA, and polymerase-chain-reaction were used.
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis
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Antibodies, Monoclonal / immunology
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Antibodies, Viral / biosynthesis
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Antibodies, Viral / immunology
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Antibody Specificity
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Antigens, Viral / immunology
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Cattle
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Cells, Cultured
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DNA, Viral / analysis
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Enzyme-Linked Immunosorbent Assay
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Epitopes / immunology
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Fluorescent Antibody Technique
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Immunoenzyme Techniques
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Lung / cytology
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Lung / embryology
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Mice
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Mice, Inbred BALB C
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Microscopy, Immunoelectron
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Orf virus / genetics
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Orf virus / immunology
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Orf virus / isolation & purification
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Parapoxvirus / genetics
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Parapoxvirus / immunology
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Parapoxvirus / isolation & purification*
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Parapoxvirus / ultrastructure
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Polymerase Chain Reaction
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Poxviridae Infections / diagnosis
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Poxviridae Infections / microbiology
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Poxviridae Infections / veterinary*
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Rabbits
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Sheep
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Antigens, Viral
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DNA, Viral
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Epitopes