The paper describes different methods for using isolated hepatocytes to compensate hepatic dysfunctions and to treat hepatic failure. Most of them are safe and produce no complications if isolated hepatocytes are separated from blood with the semipermeable membrane. The survival and functional activity of isolated hepatocytes transplanted into different places and organs are ambiguous and unequal in terms of efficiency. Search for a rational and optimal method for implantation of isolated hepatocytes, for conditions for their functioning, for combinations of different cells, for proliferation of the implanted cells is a subject of special interest and it is in progress. Many procedures have been experimentally justified and are being now used clinically to treat hepatic failure in various hepatic diseases.