Prevalence of HPV in premalignant and malignant cervical lesions in Greenland and Denmark: PCR and in situ hybridization analysis on archival material

Res Virol. Mar-Apr 1994;145(2):83-92. doi: 10.1016/s0923-2516(07)80010-0.

Abstract

Archival formalin-fixed paraffin-embedded cervical specimens from 125 women in Greenland and 139 women in Denmark who had CIN I-III or cervical cancer diagnosed between 1983 and 1987 were analysed for human papillomavirus type 16 (HPV-16) by in situ hybridization and for HPV-16, 18, 31, 33, 35 and 45 by PCR. In situ hybridization analysis showed an HPV-16 prevalence of 17% (16/95) and 23% (24/105) in the premalignant lesions from Greenland and Denmark, respectively. The HPV-16 prevalence rate in the cancer specimens was 10% (3/30) in the samples from Greenland and 29% (10/34) in the Danish specimens. A total of 82 Greenlandic and 107 Danish specimens were beta-globin-positive by PCR reaction. HPV-16-specific PCR on these samples showed 63% (34/54) of the Greenlandic and 68% (50/74) of the Danish preinvasive lesions to be positive. The corresponding HPV-16-positive rates for the invasive cancers were 82% (23/28) for Greenland and 70% (23/33) for Denmark. This study of patient samples supports our previous population-based studies in which similar HPV-detection rates were found among random samples of women from Greenland and Denmark, although Greenland has a 4-5-fold higher cervical cancer incidence.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Beta-Globulins / genetics
  • Cervical Intraepithelial Neoplasia / complications
  • Cervical Intraepithelial Neoplasia / microbiology*
  • DNA, Viral / analysis
  • Denmark / epidemiology
  • Female
  • Greenland / epidemiology
  • Humans
  • In Situ Hybridization
  • Middle Aged
  • Molecular Sequence Data
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / complications
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / microbiology*
  • Polymerase Chain Reaction
  • Prevalence
  • Tumor Virus Infections / complications
  • Tumor Virus Infections / epidemiology
  • Tumor Virus Infections / microbiology*
  • Uterine Cervical Neoplasms / complications
  • Uterine Cervical Neoplasms / microbiology*

Substances

  • Beta-Globulins
  • DNA, Viral