Cloning and characterization of cDNAs encoding two normal isoforms of bovine stem cell factor

Biochim Biophys Acta. 1994 Aug 11;1223(1):148-50. doi: 10.1016/0167-4889(94)90084-1.


The cDNA clones encoding two isoforms of bovine stem cell factor (bSCF) were obtained using reverse transcriptase-polymerase chain reaction, and their sequences were determined. The deduced amino acid sequences of the longer and shorter isoforms of bSCF consist, respectively, of 274 and 246 residues and show a high degree of identity to those of SCFs of different animal species. Northern blot analysis with the cDNA revealed the expression of a 5.8 kilobase bSCF RNA in fetal bovine tissues.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics*
  • Hematopoietic Cell Growth Factors / chemistry
  • Hematopoietic Cell Growth Factors / genetics*
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Stem Cell Factor


  • DNA, Complementary
  • Hematopoietic Cell Growth Factors
  • Stem Cell Factor

Associated data

  • GENBANK/D28934