Role of P-selectin in microvascular leukocyte-endothelial interaction in splanchnic ischemia-reperfusion

Am J Physiol. 1994 Aug;267(2 Pt 2):H622-30. doi: 10.1152/ajpheart.1994.267.2.H622.


The role of P-selectin in leukocyte-endothelial interaction after splanchnic arterial occlusion and reperfusion (SAO/R) in pentobarbital-anesthetized rats was investigated employing a P-selectin-neutralizing monoclonal antibody (i.e., MAb PB1.3). MAb PB1.3 (1 mg/kg) given intravenously to SAO/R rats just before reperfusion significantly attenuated leukocyte rolling and adherence in mesenteric postcapillary venules as observed via intravital microscopy. Likewise, ileal myeloperoxidase (MPO) activity was decreased from 4.6 +/- 0.6 in nontreated ischemic rats to 2.0 +/- 0.2 U/100 mg (P < 0.01), indicating a lesser degree of polymorphonuclear leukocyte (PMN) accumulation. A significantly lower plasma free amino-nitrogen concentration was observed in MAb PB1.3-treated rats vs. untreated (P < 0.01), suggesting decreased tissue injury after reperfusion. Immunohistochemical localization demonstrated significant expression of P-selectin in endothelial cells lining ileal postcapillary venules 30 min after reperfusion of the ischemic splanchnic circulation. Thus P-selectin appears to plays an important role in leukocyte accumulation after splanchnic ischemia-reperfusion, and the MAb PB1.3 attenuates the accumulation of PMNs in the ischemic-reperfused small bowel, resulting in reduced tissue injury.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cell Adhesion
  • Cell Adhesion Molecules / physiology
  • Endothelium, Vascular / physiopathology*
  • Hemodynamics
  • Immunohistochemistry
  • Leukocytes / physiology*
  • Male
  • Microcirculation
  • P-Selectin
  • Platelet Membrane Glycoproteins / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / physiopathology*
  • Splanchnic Circulation*


  • Antibodies, Monoclonal
  • Cell Adhesion Molecules
  • P-Selectin
  • Platelet Membrane Glycoproteins