T cells recognize a peptide derived from alpha-gliadin presented by the celiac disease-associated HLA-DQ (alpha 1*0501, beta 1*0201) heterodimer

Hum Immunol. 1994 Apr;39(4):243-52. doi: 10.1016/0198-8859(94)90267-4.


CD is unique among the HLA-associated diseases since (a) the disease-promoting agent (gliadin) is known and (b) the disease is precipitated mainly in individuals carrying a particular cis- or trans-encoded HLA-DQ heterodimer; i.e., DQ(alpha 1*0501, beta 1*0201). Further, a preponderance of gliadin-specific T cells derived from the small intestinal mucosa of CD patients are restricted by this DQ heterodimer. T-cell recognition of gliadin peptides presented by the DQ(alpha 1*0501, beta 1*0201) heterodimer may thus be of importance in CD. Here we report that a T-cell clone from a patient with CD recognizes a synthetic alpha-gliadin peptide, when presented by the cis- or trans-encoded CD-associated DQ(alpha 1*0501, beta 1*0201) heterodimer. The minimal peptide recognized by the T-cell clone corresponds to residues 31-47 of alpha-gliadin, which is included in the part of alpha-gliadin previously shown to have disease-promoting activity. When testing analogue peptides derived from other alpha-gliadin sequences, one peptide differing by one amino acid was recognized by the T-cell clone, whereas the other peptide differing by two amino acids was not recognized. Our findings demonstrate that the CD-associated DQ(alpha 1*0501, beta 1*0201) heterodimer may serve as an antigen-presenting molecule to T cells for certain gliadin peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigen Presentation / immunology*
  • Antigen-Presenting Cells / immunology
  • Celiac Disease / immunology*
  • Cell Line
  • Cells, Cultured
  • Clone Cells
  • Epitopes / immunology
  • Female
  • Gliadin / chemical synthesis
  • Gliadin / immunology*
  • HLA-DQ Antigens / genetics
  • HLA-DQ Antigens / immunology*
  • Humans
  • Immunophenotyping
  • Lymphocyte Activation / immunology
  • Male
  • Molecular Sequence Data
  • Peptides / chemical synthesis
  • Peptides / immunology*
  • T-Lymphocytes / immunology*


  • Epitopes
  • HLA-DQ Antigens
  • HLA-DQ2 antigen
  • Peptides
  • Gliadin