Immunomagnetic positive selection and colony culture of CD34+ cells from blood

J Hematother. Summer 1993;2(2):247-50. doi: 10.1089/scd.1.1993.2.247.

Abstract

Immunomagnetic separation has been used to enrich CD34-positive cells in umbilical cord blood. Cell purities were increased from 0.59% preseparation to 92.7% postseparation (n = 16) with a mean yield of 75.7%. CFU were enriched 127 fold by immunomagnetic separation. Addition of combinations of recombinant growth factors resulted in cloning efficiencies of greater than 50%.

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, CD34
  • Cell Count
  • Colony-Forming Units Assay
  • Fetal Blood / cytology*
  • Hematopoietic Cell Growth Factors / pharmacology
  • Hematopoietic Stem Cells* / drug effects
  • Humans
  • Immunomagnetic Separation* / instrumentation
  • Immunophenotyping
  • Infant, Newborn
  • Stem Cell Factor

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, CD34
  • Hematopoietic Cell Growth Factors
  • Stem Cell Factor