Identification of a ToxR-activated gene, tagE, that lies within the accessory colonization factor gene cluster of Vibrio cholerae O395

Gene. 1994 Oct 11;148(1):91-5. doi: 10.1016/0378-1119(94)90239-9.

Abstract

The nucleotide (nt) sequence has been determined for a Vibrio cholerae ToxR-activated gene designated tagE that is located within a cluster of genes required for efficient intestinal colonization. The tagE gene encompasses 909 nt and is predicted to encode a 303-amino-acid (aa) protein with an estimated molecular mass of 34,468 Da. Computer-assisted similarity searches revealed that TagE possesses aa sequence similarity with Escherichia coli OrfU and Staphylococcus simulans lysostaphin, two proteins that are involved in cell-wall biosynthesis and peptidoglycan degradation, respectively. The role, if any, that TagE plays in the accessory colonization factor phenotype is currently under investigation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Base Sequence
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Fimbriae Proteins*
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial / genetics*
  • Molecular Sequence Data
  • Multigene Family / genetics
  • RNA, Bacterial / biosynthesis
  • RNA, Messenger / biosynthesis
  • Regulon / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Transcription, Genetic
  • Vibrio cholerae / genetics*

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • RNA, Bacterial
  • RNA, Messenger
  • TagE protein, Vibrio cholerae
  • Transcription Factors
  • colonization factor antigens
  • toxR protein, Vibrio cholerae
  • toxR protein, bacteria
  • Fimbriae Proteins

Associated data

  • GENBANK/U07173