Synthetic oligonucleotides with certain palindromes stimulate interferon production of human peripheral blood lymphocytes in vitro

Jpn J Cancer Res. 1994 Aug;85(8):775-9. doi: 10.1111/j.1349-7006.1994.tb02947.x.


We studied the ability of synthetic single-stranded 30-mer oligodeoxyribonucleotides (oligoDNAs) with three different kinds of hexamer palindromic sequence to induce interferon (IFN) production of human peripheral blood lymphocytes (PBL). When PBL was cultured with oligoDNA having a palindrome of AACGTT or GACGTC, IFN activity was detected by bioassay in the culture fluid after 8 h, and the amount of IFN reached the maximum after 18 h. IFN-alpha was predominantly produced, and small amounts of IFN-beta and IFN-gamma were also found. OligoDNA with the palindrome ACCGGT had no effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cells, Cultured
  • Humans
  • Interferon Inducers / pharmacology*
  • Interferon-alpha / biosynthesis
  • Interferon-beta / biosynthesis
  • Interferon-gamma / biosynthesis
  • Interferons / biosynthesis*
  • Kinetics
  • Lymphocytes / drug effects
  • Lymphocytes / immunology
  • Lymphocytes / metabolism*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / pharmacology*
  • Time Factors


  • Interferon Inducers
  • Interferon-alpha
  • Oligodeoxyribonucleotides
  • Interferon-beta
  • Interferon-gamma
  • Interferons