Xeroderma pigmentosum group E binding factor recognizes a broad spectrum of DNA damage

Mutat Res. 1994 Oct 1;310(1):89-102. doi: 10.1016/0027-5107(94)90012-4.

Abstract

Xeroderma pigmentosum complementation group E binding factor (XPE-BF) is a damaged DNA binding protein that is deficient in a subset of patients from complementation group E of xeroderma pigmentosum. The protein recognizes various forms of DNA damage including some cyclobutane pyrimidine dimers, 6-4 photoproducts, cis-diamminedichloroplatinum(II) adducts, and single-stranded DNA. We now show that it also recognizes damage induced by nitrogen mustard; N-methyl-N'-nitro-N-nitrosoguanidine, and depurination, but has no detectable affinity for DNA adducts generated by trans-diamminedichloroplatinum(II), 4-nitroquinoline-N-oxide, 8-methoxypsoralen, or enzymatically methylated cytosine and adenine. The failure to recognize 4-nitroquinoline-N-oxide and 8-methoxypsoralen adducts is consistent with previous reports that XPE cells carry out wild-type levels of repair synthesis after DNA damage by those drugs. These results demonstrate that XPE-BF is a versatile damage recognition protein, but suggest that other proteins must contribute to the recognition of DNA lesions for the human excision repair pathway.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 4-Nitroquinoline-1-oxide / metabolism
  • 4-Nitroquinoline-1-oxide / toxicity
  • Cisplatin / metabolism
  • Cisplatin / toxicity
  • DNA / drug effects
  • DNA / metabolism
  • DNA / radiation effects
  • DNA Damage*
  • DNA Repair
  • DNA-Binding Proteins / metabolism*
  • Genetic Complementation Test
  • HeLa Cells
  • Humans
  • Mechlorethamine / metabolism
  • Mechlorethamine / toxicity
  • Methoxsalen / metabolism
  • Methoxsalen / toxicity
  • Methylnitronitrosoguanidine / metabolism
  • Methylnitronitrosoguanidine / toxicity
  • Mutagens / metabolism
  • Mutagens / toxicity
  • Ultraviolet Rays
  • Xeroderma Pigmentosum / genetics
  • Xeroderma Pigmentosum / metabolism*

Substances

  • DDB1 protein, human
  • DNA-Binding Proteins
  • Mutagens
  • Methylnitronitrosoguanidine
  • transplatin
  • Mechlorethamine
  • 4-Nitroquinoline-1-oxide
  • DNA
  • Cisplatin
  • Methoxsalen