The single pseudouridine residue in Escherichia coli 16S RNA is located at position 516

Nucleic Acids Res. 1994 Sep 11;22(18):3681-4. doi: 10.1093/nar/22.18.3681.


The number and location of pseudouridine residues in Escherichia coli 16S ribosomal RNA has been determined by a combination of direct and indirect methods. Only one residue was found, at position 516. This site is at the 5'-end of one of the three most highly conserved long sequences of this RNA molecule. A number of experimental findings have strongly implicated this loop in the fidelity of codon recognition by A-site bound tRNA. By virtue of its location, we suggest that psi 516 may also play a role in maintaining the fidelity of protein synthesis.

MeSH terms

  • Base Sequence
  • Conserved Sequence
  • Escherichia coli / chemistry*
  • Escherichia coli / genetics
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Pseudouridine / analysis*
  • Pseudouridine / genetics
  • RNA, Bacterial / chemistry
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / chemistry*
  • RNA, Ribosomal, 16S / genetics


  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Pseudouridine