Distinct structural and functional epitopes of the alpha E beta 7 integrin

Eur J Immunol. 1994 Nov;24(11):2832-41. doi: 10.1002/eji.1830241138.


Intestinal intraepithelial lymphocytes (iIEL) are predominantly CD3+, CD8+ T lymphocytes located above or adjacent to the mucosal basement membrane. Although they are positioned to interact with intercellular luminal antigen or with enterocytes, the function of iIEL remains unknown. Most (> 85%) of the iIEL express the alpha E beta 7 integrin which appears to be involved in the adhesion of lymphocytes to epithelial cells. We report the characterization of three monoclonal antibodies (mAb) termed alpha E7-1, alpha E7-2, and alpha E7-3, that react with the alpha E beta 7 integrin recognized by the previously described mAb HML-1 as demonstrated by identical sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobility and charge. Flow cytometric analysis of antibody cross-blocking indicated that these mAb recognize distinct epitopes of alpha E beta 7. While all of the mAb were capable of blocking the adhesion of cultured iIEL to a breast epithelial cell line, only HML-1 and alpha E7-1 (which recognize an identical or closely related epitope) were co-stimulatory with suboptimal concentrations of anti-CD3 mAb in inducing proliferation of cultured iIEL. Thus, these mAb appear to recognize functionally distinct epitopes of alpha E beta 7 and will be useful to study relationships between the structure and function of this integrin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Cell Adhesion
  • Cell Line
  • Epitopes*
  • Extracellular Matrix Proteins / physiology
  • Humans
  • Integrins / chemistry
  • Integrins / immunology*
  • Integrins / physiology
  • Intestinal Mucosa / immunology*
  • Lymphocyte Activation
  • Lymphocytes / chemistry
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Transforming Growth Factor beta / pharmacology


  • Antibodies, Monoclonal
  • Epitopes
  • Extracellular Matrix Proteins
  • Integrins
  • Transforming Growth Factor beta
  • integrin alphaEbeta7