Variable expression of O-antigen and the role of lipopolysaccharide as an adhesin in Aeromonas sobria

FEMS Microbiol Lett. 1994 Sep 15;122(1-2):97-101. doi: 10.1111/j.1574-6968.1994.tb07150.x.

Abstract

On initial isolation of Aeromonas sobria 3767 from a diarrhoeal stool specimen, two colony types were obtained: opaque (3767O) and translucent (3767T). Strain 3767O consistently produced lipopolysaccharide (LPS) core and O-antigen side chain, detectable by SDS-PAGE and by Western blotting with an O-antigen-specific monoclonal antibody. Strain 3767T produced LPS core but the amount of O-antigen was dependent on factors including growth medium and bacterial growth phase. Strain 3767T exhibited significantly lower levels of adhesion to HEp-2 cells than 3767O and this correlated with the level of LPS expression, with the greatest reduction (61%) at stationary phase when no LPS was detectable. The results implicate LPS as an adhesin for A. sobria 3767.

MeSH terms

  • Adhesins, Bacterial / physiology*
  • Aeromonas / classification
  • Aeromonas / growth & development
  • Aeromonas / metabolism*
  • Lipopolysaccharides / metabolism*
  • O Antigens
  • Polysaccharides, Bacterial / metabolism*
  • Species Specificity

Substances

  • Adhesins, Bacterial
  • Lipopolysaccharides
  • O Antigens
  • Polysaccharides, Bacterial