Abstract
The expedient of preparing homologous DNA samples substituted with I for G, DAP for A, or both, has been used to investigate the role of the purine 2-amino group in determining the preferred binding sites for antibiotics on DNA. The selectivity of echinomycin for CpG steps, of actinomycin for GpC steps, and of netropsin for A + T-rich tracts, is seen to be radically altered in the substituted DNA molecules.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2-Aminopurine / analogs & derivatives*
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2-Aminopurine / metabolism
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Adenine / metabolism
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Anti-Bacterial Agents / metabolism*
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Autoradiography
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Base Sequence
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DNA / chemistry
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DNA / metabolism*
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Dactinomycin / metabolism
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Deoxyribonuclease I
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Echinomycin / metabolism
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Guanine / metabolism
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Inosine / metabolism
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Molecular Sequence Data
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Netropsin / metabolism
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Nucleic Acid Conformation
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RNA, Transfer, Tyr / genetics
Substances
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Anti-Bacterial Agents
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RNA, Transfer, Tyr
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Dactinomycin
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2-Aminopurine
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2,6-diaminopurine
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Echinomycin
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Inosine
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Guanine
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Netropsin
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DNA
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Deoxyribonuclease I
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Adenine