The purine 2-amino group as a critical recognition element for binding of small molecules to DNA

Gene. 1994 Nov 4;149(1):69-79. doi: 10.1016/0378-1119(94)90414-6.

Abstract

The expedient of preparing homologous DNA samples substituted with I for G, DAP for A, or both, has been used to investigate the role of the purine 2-amino group in determining the preferred binding sites for antibiotics on DNA. The selectivity of echinomycin for CpG steps, of actinomycin for GpC steps, and of netropsin for A + T-rich tracts, is seen to be radically altered in the substituted DNA molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Aminopurine / analogs & derivatives*
  • 2-Aminopurine / metabolism
  • Adenine / metabolism
  • Anti-Bacterial Agents / metabolism*
  • Autoradiography
  • Base Sequence
  • DNA / chemistry
  • DNA / metabolism*
  • Dactinomycin / metabolism
  • Deoxyribonuclease I
  • Echinomycin / metabolism
  • Guanine / metabolism
  • Inosine / metabolism
  • Molecular Sequence Data
  • Netropsin / metabolism
  • Nucleic Acid Conformation
  • RNA, Transfer, Tyr / genetics

Substances

  • Anti-Bacterial Agents
  • RNA, Transfer, Tyr
  • Dactinomycin
  • 2-Aminopurine
  • 2,6-diaminopurine
  • Echinomycin
  • Inosine
  • Guanine
  • Netropsin
  • DNA
  • Deoxyribonuclease I
  • Adenine