Interferon-gamma regulation of interleukin 6 in monocytic cells

Am J Physiol. 1994 Nov;267(5 Pt 1):L564-8. doi: 10.1152/ajplung.1994.267.5.L564.


The regulation of interleukin 6 (IL-6) is dependent on many factors that include numerous stimuli such as lipopolysaccharide (LPS), viruses, and other cytokines. These studies demonstrate the ability of interferon-gamma (IFN-gamma) to significantly enhance IL-6 mRNA and protein production in LPS-stimulated monocytes and THP-1 cells. IL-6 protein production was increased sevenfold in LPS-stimulated cells with the addition of IFN-gamma. IL-6 mRNA production was increased in a dose-dependent fashion up to 15-fold in LPS-stimulated cells with the addition of IFN-gamma. The enhanced production of IL-6 mRNA that occurs with the addition of IFN-gamma to LPS-stimulated monocytic cells was due to increased transcription of IL-6 mRNA. The ability of IFN-gamma to enhance IL-6 production may play an important role in many inflammatory processes.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antigens, CD / metabolism
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • Half-Life
  • Humans
  • Interferon-gamma / pharmacology*
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism*
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides / pharmacology
  • Monocytes / metabolism*
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • Transcription, Genetic / drug effects
  • Tumor Cells, Cultured


  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Interleukin-6
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • RNA, Messenger
  • Interferon-gamma