Inhibition of purified nitric oxide synthase from rat cerebellum and macrophage by L-arginine analogs

Arch Biochem Biophys. 1994 Dec;315(2):213-8. doi: 10.1006/abbi.1994.1492.

Abstract

The inhibition of nitric oxide synthase (NOS) activity by a variety of L-arginine-related compounds has been investigated. The inhibitory properties of NG-amino-, NG-methyl-, NG-hydroxy-, NG-ethyl-, NG-allyl-, NG, NG-dimethyl-, NG-methoxy-L-arginine, and several other L-arginine derivatives were compared in NOS purified from both macrophage and rat cerebellum. Also, these compounds were tested for their potential as alternate substrates by determining their ability to elicit NADPH consumption by NOS. NG-Methoxy-L-arginine appears to be an alternate substrate for NOS, whereas most other L-arginine analogs, except for the biosynthetic intermediate NG-hydroxy-L-arginine, do not elicit significant enzyme turnover.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Oxidoreductases / antagonists & inhibitors*
  • Animals
  • Arginine / analogs & derivatives*
  • Arginine / metabolism
  • Cerebellum / enzymology*
  • Hydrogen Peroxide / metabolism
  • In Vitro Techniques
  • Macrophages / enzymology*
  • NADP / metabolism
  • Nitric Oxide Synthase
  • Rats
  • Structure-Activity Relationship

Substances

  • NADP
  • Arginine
  • Hydrogen Peroxide
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases